The total oil carbon of Wuluqiao oil seepage in Awati Sag has a comparatively heavy isotope of -29.4‰, which fells at the area of high maturity oil in the F1-F2 plate. The oil of the seepage shows a high MPR of 2.03, which means a higher maturity than those of other high mature condensate oil and crude oil having found in Tarim Basin. The distributions of terpane and gonane are characteristic of low abundance of C29Ts and rearranged hopane, higher abundance of gammacerane，medium-high abundance of C28-gonane, and low abundance of diasteranes, which are similar with those of the oil from Cambrian source rock but obviously different from those of oil from the Carboniferous-Permian, Triassic and Middle-Upper Ordovician source rocks in this sag and its periphery. According to multidisciplinary analysis, it concludes that the oil of Wuluqiao oil seepage is original from Cambrian source rock during the late period of hydrocarbon generation.