A kinetic simulating experiment of hydrocarbon generation was carried out on Lower Palaeozoic marine carbonate rocks in the Jiyang Depression, Bohaiwan Basin. The kinetic parameters of hydrocarbon generation were determined and these parameters were used to extrapolate the hydrocarbon-generating quantity in the carbonate rocks. Based on it, the kinetics of the secondary hydrocarbon generation and the hydrocarbon generation history was also simulated to ascertain the potential of hydrocarbon generation from the Lower Palaeozoic carbonate source rocks. Since high geothermal gradient and high earth thermal current were widely distributed during Yenshanian movment, the palaeo-geothermal field is the key kinetic factor of secondary hydrocarbon generation in Lower Palaeozoic carbonate rocks. The determination of vitrinite reflectance by EasyRo model indicates that the values of REasy 0.5,~0.87, correspond to the first hydrocarbon generation in the carbonate rocks and the values of REasy 0.87,~2.30, do to the secondary hydrocarbon generation, which is the main stage of hydrocarbon generation of the carbonate source rocks in Jiyang Depression. Thermally-degrading gaseous hydrocarbons (C1,C2,C3 and C4-5) in Lower Palaeozoic carbonate rocks mostly generated between 130 Ma and 100Ma (corresponding to REasy 0.8,~2.3,) and light hydrocarbons(C7~C13) and heavy hydrocarbons(C13+) mostly generated between 140Ma and 110 Ma (corresponding to REasy 0.5,~1.5,).