It is found that beside the light crude oil from lower Ordovician Dawan rock in Well Hu-47 in Kaili area (southeastern Guizhou), abundant residual seepage oils are present in middle Ordovician Dawan and lower Ordovician Honghuayan limestone fractures and vugs at some oil seepage sites in this area. The oil samples from the Well Hu-47 and these oil seepages are analyzed by GC, GC-MS and GC-IRMS. The molecule geochemical characteristics and organic mater maturity indicate that the kerogen in both crude oil and seepage oils came from algae that formed in reducing environment. Methylphenanthrene indice (MPI) reveal that the crude oil is mature and the seepage oils are higher mature. There is the lower Cambrian Niutitang argillaceous source rock in this area. According to the correlation of the oil-source rock through carbon isotope testing to individual hydrocarbon, it is confirmed that both of the crude oil and the seepage oils derived from the identical Niutitang source rock but they formed in different phases.