Take the Carboniferous hydrocarbon accumulation of Bashituo structure belt of Markit slope in Tarim Basin as an example, it is identified four kinds of oil inclusions which developed in Carboniferous reservoir with different fluorescent color, from yellow, yellow-green to blue-green and blue-white. It is shown that the grade of maturity of oil charging in the oil inclusions is gradually increased. The property parameters of main peak wavelength λmax, red-green entropy Q and QF535 were calculated by using the fluorescence spectra of crude oil inclusions. Based on the characteristics of four different types of correlation relationship between λmax and QF535 , it is concluded that the Carboniferous reservoir has experienced four stages of hydrocarbon charging in the geological history, and it also reflected the four stages of oil charging with different thermal evolution according to the homogenization temperature of fluid inclusions. It is inferred that the late Hercynian is the important hydrocarbon accumulation stage in Bashituo structure belt.