In recent years, several giant gas reservoirs have been found in the deep-water area of offshore Mozambique in East Africa, making this area the focus of gas exploration in the world. However, the exploration degree in this area is low, and the research on deep-water sedimentary system is at a low level, which restricts the fine petroleum exploration in this area. Based on core, logging, and 3D seismic data, the sequence stratigraphic framework of Mozambique sea area is established by integrating the key technologies of deep-water oil and gas exploration, such as deep-water sedimentary sequence stratigraphic identification and division method, deep-water sedimentary structural unit comprehensive description technology, deep-water sedimentary reservoir prediction and fluid identification technology, and deep-water sedimentary reservoir 3D geological modeling technology. A comprehensive description chart of deep-water sedimentary structural elements was formed, which was used for effectively predicting the distribution of deep-water sedimentary reservoirs and fluid distribution. The research results provide technical support for reserve evaluation and well deployment, boost the development of trillions of cubic meters of giant gas fields in Mozambique, and achieve good application results.